Type: HPLC Application #: 102800
Detection: radioactivity Conditions: eluent A: water eluent B: 3.4M ammonium formate, adjusted to pH 3.8 with H3PO4 6min isocratic 100% A, then gradient to 15% B in 24min, finally to 60% B in 10min flow rate 2ml/min Authors: Maly Source: Univ Innsbruck Austria 1992
Details
Legend: a) Separation of radioactively labelled inositol phosphates from NIH 3T3
fibroblasts 30sec after stimulation with 2µM bombesin, 1500µl injected.